Category: Incremento de la productividad
Location: Estación Experimental Litoral Sur
Year: 2017
Link: Link
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Passion fruit (Passiflora edulis) is a native of tropical America and is cultivated throughout tropical and subtropical regions around the world. It belongs to the family Passifloraceae and is commonly known as yellow passion fruit, maracuyá, yellow granadilia, and pommelianejaume (Sunitha and Devaki 2009). Ecuador is the main exporter of maracuyá pulp in the world. It is grown in an area of 13,389 ha, with a production of 58,522 t (MAGAP 2016). During September 2015, mosaic, blistering, curling, and necrosis symptoms were seen on passion fruit plants in Quevedo, Los Ríos, Ecuador. Ten seedlings of Cucurbita pepo (squash) were inoculated with sap from symptomatic passion fruit leaves using 0.01 M phosphate buffer (pH 7.0). Vein clearing and blistering symptoms were observed 11 days post inoculation (dpi). Field-infected and sap-inoculated passion fruit and C. pepo samples showed positive reactions against Cucumber mosaic virus (CMV) polyclonal antibodies using TAS-ELISA (Agdia, U.S.A.). The sap-inoculated passion fruit leaves were also positive for CMV in TAS-ELISA. Total RNA was isolated from a field-infected passion fruit leaf sample using Trizol (Sigma). In reverse transcription (RT)-PCR, a DNA fragment of ∼500 bp was amplified using specific primers for the coat protein (CP) gene of CMV (Liu et al. 2012). The amplicon was sequenced and deposited in NCBI GenBank (accession no. KX470408). Sequence comparison (BioEdit v. 7.05) of the CP gene of the Ecuadorian isolate with other CMV isolates (KT302171-China, DQ302719-China, AJ585521-Australia, FJ403473-China, JN559384-China, EU250361-Taiwan, EF213025-China, AJ871492-Taiwan, AF368192-China, KC527745-South Korea, GU111229-India, AJ829779-Spain, KR535607-South Korea, AF418577-Brazil, KU144674-Mexico, GU453918-U.S.A., AY429432-China, and FN257306-Italy) showed maximum identity of 99.4% at the nucleotide level and 99.1% identity at the amino acid level with KT302171-China. CMV belongs to the genus Cucumovirus and family Bromoviridae. It is transmitted by aphids in a nonpersistent manner and has a wide host range that includes over 1,200 species belonging to 100 plant families. CMV causes significant economic losses in vegetable crops, horticulture crops, and weed plants (Zitter and Murphy 2009). To our knowledge, this is the first report of CMV on passion fruit in Ecuador


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